Structure-seq2: sensitive and accurate genome-wide profiling of RNA structure in vivo.

ABSTRACT: RNA serves many functions in biology such as splicing, temperature sensing, and innate immunity. These functions are often determined by the structure of RNA. There is thus a pressing need to understand RNA structure and how it changes during diverse biological processes both in vivo and genome-wide. Here, we present Structure-seq2, which provides nucleotide-resolution RNA structural information in vivo and genome-wide. This optimized version of our original Structure-seq method increases sensitivity by at least 4-fold and improves data quality by minimizing formation of a deleterious by-product, reducing ligation bias, and improving read coverage. We also present a variation of Structure-seq2 in which a biotinylated nucleotide is incorporated during reverse transcription, which greatly fa

SUBMITTER: Ritchey LE 

PROVIDER: S-EPMC5737731 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature