Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities.

ABSTRACT: Pervasive transcription of genomes generates multiple classes of non-coding RNAs. One of these classes are stable long non-coding RNAs which overlap coding genes in antisense direction (asRNAs). The function of such asRNAs is not fully understood but several cases of antisense-dependent gene expression regulation affecting the overlapping genes have been demonstrated. Using high-throughput yeast genetics and a limited set of four growth conditions we previously reported a regulatory function for ∼25% of asRNAs, most of which repress the expression of the sense gene. To further explore the roles of asRNAs we tested more conditions and identified 15 conditionally antisense-regulated genes, 6 of which exhibited antisense-dependent enhancement of gene expression. We focused on the sporulation-

SUBMITTER: Bunina D 

PROVIDER: S-EPMC5737743 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature