Nucleic acid binding proteins affect the subcellular distribution of phosphorothioate antisense oligonucleotides.

ABSTRACT: Antisense oligonucleotides (ASOs) are versatile tools that can regulate multiple steps of RNA biogenesis in cells and living organisms. Significant improvements in delivery, potency, and stability have been achieved through modifications within the oligonucleotide backbone, sugar and heterocycles. However, these modifications can profoundly affect interactions between ASOs and intracellular proteins in ways that are only beginning to be understood. Here, we report that ASOs with specific backbone and sugar modifications can become localized to cytoplasmic ribonucleoprotein granules such as stress granules and those seeded by the aggregation of specific ASO-binding proteins such as FUS/TLS (FUS) and PSF/SFPQ (PSF). Further investigation into the basis for ASO-FUS binding illustrated the imp

SUBMITTER: Bailey JK 

PROVIDER: S-EPMC5737868 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature