Differentiation of nonhuman primate pluripotent stem cells into functional keratinocytes.

ABSTRACT:

Background

Epidermal grafting using cells derived from pluripotent stem cells will change the face of this side of regenerative cutaneous medicine. To date, the safety of the graft would be the major unmet deal in order to implement long-term skin grafting. In this context, experiments on large animals appear unavoidable to assess this question and possible rejection. Cellular tools for large animal models should be constructed.

Methods

In this study, we generated monkey pluripotent stem cell-derived keratinocytes and evaluated their capacities to reconstruct an epidermis, in vitro as well as in vivo.

Results

Monkey pluripotent stem cells were differentiated efficiently into keratinocytes able to reconstruct fully epidermis presenting a low level of major histocompati

SUBMITTER: Domingues S 

PROVIDER: S-EPMC5738144 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature