Machine Learning for Nuclear Mechano-Morphometric Biomarkers in Cancer Diagnosis.

ABSTRACT: Current cancer diagnosis employs various nuclear morphometric measures. While these have allowed accurate late-stage prognosis, early diagnosis is still a major challenge. Recent evidence highlights the importance of alterations in mechanical properties of single cells and their nuclei as critical drivers for the onset of cancer. We here present a method to detect subtle changes in nuclear morphometrics at single-cell resolution by combining fluorescence imaging and deep learning. This assay includes a convolutional neural net pipeline and allows us to discriminate between normal and human breast cancer cell lines (fibrocystic and metastatic states) as well as normal and cancer cells in tissue slices with high accuracy. Further, we establish the sensitivity of our pipeline by detecting sub

SUBMITTER: Radhakrishnan A 

PROVIDER: S-EPMC5738417 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature