Challenges in Estimating the Motility Parameters of Single Processive Motor Proteins.

ABSTRACT: Cytoskeletal motor proteins are essential to the function of a wide range of intracellular mechano-systems. The biophysical characterization of their movement along their filamentous tracks is therefore of large importance. Toward this end, single-molecule, in vitro stepping-motility assays are commonly used to determine motor velocity and run length. However, comparing results from such experiments has proved difficult due to influences from variations in the experimental conditions and the data analysis methods. Here, we investigate the movement of fluorescently labeled, processive, dimeric motor proteins and propose a unified algorithm to correct the measurements for finite filament length as well as photobleaching. Particular emphasis is put on estimating the statistical errors associa

SUBMITTER: Ruhnow F 

PROVIDER: S-EPMC5738525 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature