Adenosine A_2A receptor promotes collagen type III synthesis via β-catenin activation in human dermal fibroblasts.

ABSTRACT:

Background and purpose

Adenosine A_2A receptor stimulation promotes the synthesis of collagen type I and type III (Col1 and Col3), mediators of fibrosis and scarring. The A_2A receptor modulates collagen balance via cAMP/PKA/p38-MAPK/Akt pathways. Wnt signalling is important in fibrosis and the cAMP and Wnt pathways converge. Because the A_2A receptor is Gs-linked and increases cAMP, we determined whether A_2A receptors and Wnt signalling interact.

Experimental approach

Total β-catenin, de-phosphorylated β-catenin (canonical activation, de-phospho β-catenin) and phosphorylated β-catenin at Ser⁵⁵² (non-canonical activation, p-Ser⁵⁵² β-catenin) levels were determined in primary human dermal fibroblasts, cytosol and

SUBMITTER: Shaikh G 

PROVIDER: S-EPMC5738670 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature