Bronchial biopsy specimen as a surrogate for DNA methylation analysis in inoperable lung cancer.

ABSTRACT:

Background

This study was aimed at understanding whether bronchial biopsy specimen can be used as a surrogate for DNA methylation analysis in surgically resected lung cancer.

Methods

A genome-wide methylation was analyzed in 42 surgically resected tumor tissues, 136 bronchial washing, 12 sputum, and 8 bronchial biopsy specimens using the Infinium HumanMethylation450 BeadChip, and models for prediction of lung cancer were evaluated using TCGA lung cancer data.

Results

Four thousand seven hundred and twenty-six CpGs (P < 1.0E-07) that were highly methylated in tumor tissues were identified from 42 lung cancer patients. Ten CpGs were selected for prediction of lung cancer. Genes including the 10 CpGs were classified into three categories: (i) transcription (HOX

SUBMITTER: Um SW 

PROVIDER: S-EPMC5738682 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature