Visualizing Bacterial Colony Morphologies Using Time-Lapse Imaging Chamber MOCHA.

ABSTRACT: Capturing microbial growth on a macroscopic scale is of great importance to further our understanding of microbial life. However, methods for imaging microbial life on a scale of millimeters to centimeters are often limited by designs that have poor environmental control, resulting in dehydration of the agar plate within just a few days. Here, we created MOCHA (microbial chamber), a simple but effective chamber that allows users to study microbial growth for extended periods (weeks) in a stable environment. Agar hydration is maintained with a double-decker design, in which two glass petri dishes are connected by a wick, allowing the lower plate to keep the upper plate hydrated. This flexible chamber allows the observation of a variety of microbiological phenomena, such as the growth and de

SUBMITTER: Penil Cobo M 

PROVIDER: S-EPMC5738739 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature