A multiplex marker set for microsatellite typing and sexing of sooty terns Onychoprion fuscatus.

ABSTRACT:

Objectives

Seabirds have suffered dramatic population declines in recent decades with one such species being the sooty tern Onychoprion fuscatus. An urgent call to re-assess their conservation status has been made given that some populations, such as the one on Ascension Island, South Atlantic, have declined by over 80% in three generations. Little is known about their population genetics, which would aid conservation management through understanding ecological processes and vulnerability to environmental change. We developed a multiplex microsatellite marker set for sooty terns including sex-typing markers to assist population genetics studies.

Results

Fifty microsatellite loci were isolated and tested in 23 individuals from Ascension Island. Thirty-one were polymorphic and

SUBMITTER: Garrett LJH 

PROVIDER: S-EPMC5738816 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature