Promoting the expansion and function of human corneal endothelial cells with an orbital adipose-derived stem cell-conditioned medium.

ABSTRACT:

Background

Corneal endothelial dysfunction causes severe impairment of vision. The only solution is corneal transplantation. However, this treatment is hampered by a worldwide shortage of donor corneas. New therapies may replace the conventional donor corneal transplantation alongside the developments in regenerative medicine and tissue engineering, but sufficient functional corneal endothelial cells (CECs) are essential. The aim of this study was to promote the expansion and function of human corneal endothelial cells (HCECs) in vitro and in vivo.

Methods

The phenotypes of human orbital adipose-derived stem cells (OASCs) were detected by flow cytometry and immunofluorescence. HCECs were isolated and cultured using a conditioned medium obtained from OASCs (OASC-CM) in vitro.

SUBMITTER: Sun P 

PROVIDER: S-EPMC5738836 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature