A MBD-seq protocol for large-scale methylome-wide studies with (very) low amounts of DNA.

ABSTRACT: We recently showed that, after optimization, our methyl-CpG binding domain sequencing (MBD-seq) application approximates the methylome-wide coverage obtained with whole-genome bisulfite sequencing (WGB-seq), but at a cost that enables adequately powered large-scale association studies. A prior drawback of MBD-seq is the relatively large amount of genomic DNA (ideally >1 µg) required to obtain high-quality data. Biomaterials are typically expensive to collect, provide a finite amount of DNA, and may simply not yield sufficient starting material. The ability to use low amounts of DNA will increase the breadth and number of studies that can be conducted. Therefore, we further optimized the enrichment step. With this low starting material protocol, MBD-seq performed equally well, or better, th

SUBMITTER: Aberg KA 

PROVIDER: S-EPMC5739096 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature