Disease relevant modifications of the methylome and transcriptome by particulate matter (PM_2.5) from biomass combustion.

ABSTRACT: Exposure to particulate matter (PM) is recognized as a major health hazard, but molecular responses are still insufficiently described. We analyzed the epigenetic impact of ambient PM_2.5 from biomass combustion on the methylome of primary human bronchial epithelial BEAS-2B cells using the Illumina HumanMethylation450 BeadChip. The transcriptome was determined by the Affymetrix HG-U133 Plus 2.0 Array. PM_2.5 induced genome wide alterations of the DNA methylation pattern, including differentially methylated CpGs in the promoter region associated with CpG islands. Gene ontology analysis revealed that differentially methylated genes were significantly clustered in pathways associated with the extracellular matrix, cellular adhesion, function of GTPases, and responses to ex

SUBMITTER: Heßelbach K 

PROVIDER: S-EPMC5739103 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature