Interplay between alternatively spliced Tissue Factor and full length Tissue Factor in modulating coagulant activity of endothelial cells.

ABSTRACT:

Background

Full length Tissue factor (flTF) is a key player in hemostasis and also likely contributes to venous thromboembolism (VTE), the third most common cardiovascular disease. flTF and its minimally coagulant isoform, alternatively spliced TF (asTF), have been detected in thrombi, suggesting participation of both isoforms in thrombogenesis, but data on participation of asTF in hemostasis is lacking. Therefore, we assessed the role of asTF in flTF cofactor activity modulation, using a co-expression system.

Objective

To investigate the interplay between flTF and asTF in hemostasis on endothelial cell surface.

Methods

Immortalized endothelial (ECRF) cells were adenovirally transduced to express asTF and flTF, after which flTF cofactor activity was measured on cells

SUBMITTER: Unlu B 

PROVIDER: S-EPMC5739307 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature