Quantitative imaging of deuterated metabolic tracers in biological tissues with nanoscale secondary ion mass spectrometry.

ABSTRACT: In the field of secondary ion mass spectrometry at nanometer scale (NanoSIMS), configuration of parallel detectors to routinely measure isotope ratios in sub-100 nm domains brings classical stable isotope tracer studies from the whole tissue level down to the suborganelle level. Over the past decade, the marriage of stable isotope tracers with NanoSIMS has been applied to a range of fundamental biological questions that were largely inaccessible by other means. Although multiplexed measurement of different stable isotope tracers is feasible, in practice there remains a gap in the current analytical capacity to efficiently measure stable isotopes commonly utilized in tracer studies. One such example is the measurement of deuterated tracers. The most obvious approach to measuring deuterium/h

SUBMITTER: Guillermier C 

PROVIDER: S-EPMC5739342 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature