A novel cell exclusion zone assay with a barrier made from room temperature vulcanizing silicone rubber.

ABSTRACT:

Objective

To examine the usefulness of room temperature vulcanizing (RTV) silicone rubber as a barrier material for cell exclusion zone assays.

Methods

We created barriers using three types of RTV silicone rubber with differing viscosities. We then assessed the adherence of these barriers to culture dishes and their ease of removal from the dishes. We tested the effect of the newly created barriers on the extracellular matrix (ECM) protein fibronectin by attaching and then removing them from fibronectin-coated culture dishes. We also conducted cell exclusion zone assays with MIO-M1 cells using this new barrier in order to measure cell migration. We used real time reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical staining to measure the effect o

SUBMITTER: Shiode Y 

PROVIDER: S-EPMC5739469 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature