Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells.

ABSTRACT:

Background

Giardia intestinalis is a non-invasive protozoan parasite that causes giardiasis in humans, the most common form of parasite-induced diarrhea. Disease mechanisms are not completely defined and very few virulence factors are known.

Methodology

To identify putative virulence factors and elucidate mechanistic pathways leading to disease, we have used proteomics to identify the major excretory-secretory products (ESPs) when Giardia trophozoites of WB and GS isolates (assemblages A and B, respectively) interact with intestinal epithelial cells (IECs) in vitro.

Findings

The main parts of the IEC and parasite secretomes are constitutively released proteins, the majority of which are associated with metabolism but several proteins are released in response to their

SUBMITTER: Ma'ayeh SY 

PROVIDER: S-EPMC5739509 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature