Using cellular fitness to map the structure and function of a major facilitator superfamily effluxer.

ABSTRACT: The major facilitator superfamily (MFS) effluxers are prominent mediators of antimicrobial resistance. The biochemical characterization of MFS proteins is hindered by their complex membrane environment that makes in vitro biochemical analysis challenging. Since the physicochemical properties of proteins drive the fitness of an organism, we posed the question of whether we could reverse that relationship and derive meaningful biochemical parameters for a single protein simply from fitness changes it confers under varying strengths of selection. Here, we present a physiological model that uses cellular fitness as a proxy to predict the biochemical properties of the MFS tetracycline efflux pump, TetB, and a family of single amino acid variants. We determined two lumped biochemical para

SUBMITTER: Perez AM 

PROVIDER: S-EPMC5740499 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature