Proteomics of phosphorylation and protein dynamics during fertilization and meiotic exit in the Xenopus egg.

ABSTRACT: Fertilization releases the meiotic arrest and initiates the events that prepare the egg for the ensuing developmental program. Protein degradation and phosphorylation are known to regulate protein activity during this process. However, the full extent of protein loss and phosphoregulation is still unknown. We examined absolute protein and phosphosite dynamics of the fertilization response by mass spectrometry-based proteomics in electroactivated eggs. To do this, we developed an approach for calculating the stoichiometry of phosphosites from multiplexed proteomics that is compatible with dynamic, stable, and multisite phosphorylation. Overall, the data suggest that degradation is limited to a few low-abundance proteins. However, this degradation promotes extensive dephosphorylation that oc

SUBMITTER: Presler M 

PROVIDER: S-EPMC5740657 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature