The potential of circulating tumor DNA methylation analysis for the early detection and management of ovarian cancer.

ABSTRACT:

Background

Despite a myriad of attempts in the last three decades to diagnose ovarian cancer (OC) earlier, this clinical aim still remains a significant challenge. Aberrant methylation patterns of linked CpGs analyzed in DNA fragments shed by cancers into the bloodstream (i.e. cell-free DNA) can provide highly specific signals indicating cancer presence.

Methods

We analyzed 699 cancerous and non-cancerous tissues using a methylation array or reduced representation bisulfite sequencing to discover the most specific OC methylation patterns. A three-DNA-methylation-serum-marker panel was developed using targeted ultra-high coverage bisulfite sequencing in 151 women and validated in 250 women with various conditions, particularly in those associated with high CA125 levels (endom

SUBMITTER: Widschwendter M 

PROVIDER: S-EPMC5740748 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature