Profiling soil microbial communities with next-generation sequencing: the influence of DNA kit selection and technician technical expertise.

ABSTRACT: Structure and diversity of microbial communities are an important research topic in biology, since microbes play essential roles in the ecology of various environments. Different DNA isolation protocols can lead to data bias and can affect results of next-generation sequencing. To evaluate the impact of protocols for DNA isolation from soil samples and also the influence of individual handling of samples, we compared results obtained by two researchers (R and T) using two different DNA extraction kits: (1) MO BIO PowerSoil^® DNA Isolation kit (MO_R and MO_T) and (2) NucleoSpin^® Soil kit (MN_R and MN_T). Samples were collected from six different sites on Okinawa Island, Japan. For all sites, differences in the results of microbial composition analyses (bacteria, archaea

SUBMITTER: Soliman T 

PROVIDER: S-EPMC5740954 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature