Quantitative RNA-seq meta-analysis of alternative exon usage in C. elegans.

ABSTRACT: Almost 20 years after the completion of the C. elegans genome sequence, gene structure annotation is still an ongoing process with new evidence for gene variants still being regularly uncovered by additional in-depth transcriptome studies. While alternative splice forms can allow a single gene to encode several functional isoforms, the question of how much spurious splicing is tolerated is still heavily debated. Here we gathered a compendium of 1682 publicly available C. elegans RNA-seq data sets to increase the dynamic range of detection of RNA isoforms, and obtained robust measurements of the relative abundance of each splicing event. While most of the splicing reads come from reproducibly detected splicing events, a large fraction of purported junctions is only supported b

SUBMITTER: Tourasse NJ 

PROVIDER: S-EPMC5741048 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature