Salivary extracellular RNA biomarkers for insulin resistance detection in hispanics.

ABSTRACT:

Aims

Insulin resistance (IR) detection is challenging and no test is currently used in clinical practice. We developed salivary biomarkers that could be used for IR detection.

Methods

We collected saliva from 186 healthy and 276 pre-diabetic participants, divided them into high and low IR groups based on a HOMA cutoff of 2.5. We profiled extracellular transcriptome by microarray in saliva supernatant from 23 high IR and 15 low IR participants, and pre-validated the top ten extracellular mRNA (exRNA) markers in a new cohort of 40 high and 40 low IR participants. A prediction panel was then built and validated in an independent cohort of 149 high and 195 low IR participants.

Results

Transcriptomic analyses identified 42 exRNA candidates differentially present in saliva

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5741087 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature