A multiplex real-time PCR for the detection and differentiation of Campylobacter phages.

ABSTRACT: Campylobacter jejuni and C. coli are important food-borne pathogens that are widespread in animal husbandry. To combat Campylobacter along the food chain, the application of lytic phages has been shown to be a promising tool. Campylobacter phages are currently classified into three groups, of which group II and group III phages are the most common. Members of each group are closely related, whereas the two groups share only little DNA similarity. Moreover, while group III phages are specific for C. jejuni, group II phages additionally infect C. coli. Phage cocktails intended to be used for applications should be composed of various phages that differ in their host range and growth kinetics. The isolation of phages is generally performed by plaque assays. This approach has the limitation th

SUBMITTER: Jackel C 

PROVIDER: S-EPMC5741259 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature