Mechanisms of sphingosine-1-phosphate-mediated vasoconstriction of rat afferent arterioles.

ABSTRACT:

Aim

Sphingosine-1-phosphate (S1P) influences resistance vessel function and is implicated in renal pathological processes. Previous studies revealed that S1P evoked potent vasoconstriction of the pre-glomerular microvasculature, but the underlying mechanisms remain incompletely defined. We postulated that S1P-mediated pre-glomerular microvascular vasoconstriction involves activation of voltage-dependent L-type calcium channels (L-VDCC) and the rho/rho kinase pathway.

Methods

Afferent arteriolar reactivity was assessed in vitro using the blood-perfused rat juxtamedullary nephron preparation, and diameter was measured during exposure to physiological and pharmacological agents.

Results

Exogenous S1P (10^-9 -10^-5 mol L^-1 ) evoked concentra

SUBMITTER: Guan Z 

PROVIDER: S-EPMC5741527 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature