AraBAD Based Toolkit for Gene Expression and Metabolic Robustness Improvement in Synechococcus elongatus.

ABSTRACT: As a novel chemical production platform, controllable and inducible modules in Synechococcus elongatus plus the ability of working in diurnal conditions are necessary. To the endeavors, inducible promoters, such as P_Trc, have been refined from Escherichia coli, but the inducer isopropyl-β-D-thiogalactoside may cause several side-effects. Meanwhile, to promote the efficiency, photomixotrophic cultivation has been applied in S. elongatus with the additional organic carbon sources. In this study, we developed L-arabinose based modules consisted of both the P_BAD inducible promoter and the metabolism of L-arabinose in S. elongatus, since L-arabinose is an ideal heterologous feedstock for its availability and economic and environmental benefits. As expected, we achieved hom

SUBMITTER: Cao YQ 

PROVIDER: S-EPMC5741739 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature