Cellular and Molecular Identity of Tumor-Associated Macrophages in Glioblastoma.

ABSTRACT: In glioblastoma (GBM), tumor-associated macrophages (TAM) represent up to one half of the cells of the tumor mass, including both infiltrating macrophages and resident brain microglia. In an effort to delineate the temporal and spatial dynamics of TAM composition during gliomagenesis, we used genetically engineered and GL261-induced mouse models in combination with CX3CR1^GFP/WT;CCR2^RFP/WT double knock-in mice. Using this approach, we demonstrated that CX3CR1^LoCCR2^Hi monocytes were recruited to the GBM, where they transitioned to CX3CR1^HiCCR2^Lo macrophages and CX3CR1^HiCCR2^- microglia-like cells. Infiltrating macrophages/monocytes constituted approximately 85% of the total TAM population, with resident mi

SUBMITTER: Chen Z 

PROVIDER: S-EPMC5741820 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature