A next generation sequencing based approach to identify extracellular vesicle mediated mRNA transfers between cells.

ABSTRACT:

Background

Exosomes and other extracellular vesicles (EVs) have emerged as an important mechanism of cell-to-cell communication. However, previous studies either did not fully resolve what genetic materials were shuttled by exosomes or only focused on a specific set of miRNAs and mRNAs. A more systematic method is required to identify the genetic materials that are potentially transferred during cell-to-cell communication through EVs in an unbiased manner.

Results

In this work, we present a novel next generation of sequencing (NGS) based approach to identify EV mediated mRNA exchanges between co-cultured adipocyte and macrophage cells. We performed molecular and genomic profiling and jointly considered data from RNA sequencing (RNA-seq) and genotyping to track the "sequence

SUBMITTER: Yang J 

PROVIDER: S-EPMC5741891 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature