Comparison of multiple transcriptomes exposes unified and divergent features of quiescent and activated skeletal muscle stem cells.

ABSTRACT:

Background

Skeletal muscle satellite (stem) cells are quiescent in adult mice and can undergo multiple rounds of proliferation and self-renewal following muscle injury. Several labs have profiled transcripts of myogenic cells during the developmental and adult myogenesis with the aim of identifying quiescent markers. Here, we focused on the quiescent cell state and generated new transcriptome profiles that include subfractionations of adult satellite cell populations, and an artificially induced prenatal quiescent state, to identify core signatures for quiescent and proliferating.

Methods

Comparison of available data offered challenges related to the inherent diversity of datasets and biological conditions. We developed a standardized workflow to homogenize the normalization

SUBMITTER: Pietrosemoli N 

PROVIDER: S-EPMC5741941 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature