Donepezil suppresses intracellular Ca2+ mobilization through the PI3K pathway in rodent microglia.

ABSTRACT: BACKGROUND:Microglia are resident innate immune cells which release many factors including proinflammatory cytokines or nitric oxide (NO) when they are activated in response to immunological stimuli. Pathophysiology of Alzheimer's disease (AD) is related to the inflammatory responses mediated by microglia. Intracellular Ca2+ signaling is important for microglial functions such as release of NO and cytokines. In addition, alteration of intracellular Ca2+ signaling underlies the pathophysiology of AD, while it remains unclear how donepezil, an acetylcholinesterase inhibitor, affects intracellular Ca2+ mobilization in microglial cells. METHODS:We examined whether pretreatment with donepezil affects the intracellular Ca2+ mobilization using fura-2 imaging and tested the effects of donepezil on

SUBMITTER: Haraguchi Y 

PROVIDER: S-EPMC5741946 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature