Single-cell analysis of experience-dependent transcriptomic states in the mouse visual cortex.

ABSTRACT: Activity-dependent transcriptional responses shape cortical function. However, a comprehensive understanding of the diversity of these responses across the full range of cortical cell types, and how these changes contribute to neuronal plasticity and disease, is lacking. To investigate the breadth of transcriptional changes that occur across cell types in the mouse visual cortex after exposure to light, we applied high-throughput single-cell RNA sequencing. We identified significant and divergent transcriptional responses to stimulation in each of the 30 cell types characterized, thus revealing 611 stimulus-responsive genes. Excitatory pyramidal neurons exhibited inter- and intralaminar heterogeneity in the induction of stimulus-responsive genes. Non-neuronal cells showed clear transcripti

SUBMITTER: Hrvatin S 

PROVIDER: S-EPMC5742025 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature