Astrocytic Lrp4 (Low-Density Lipoprotein Receptor-Related Protein 4) Contributes to Ischemia-Induced Brain Injury by Regulating ATP Release and Adenosine-A_2AR (Adenosine A2A Receptor) Signaling.

ABSTRACT:

Background and purpose

Lrp4 (low-density lipoprotein receptor-related protein 4) is predominantly expressed in astrocytes, where it regulates glutamatergic neurotransmission by suppressing ATP release. Here, we investigated Lrp4's function in ischemia/stroke-induced brain injury response, which includes glutamate-induced neuronal death and reactive astrogliosis.

Methods

The brain-specific Lrp4 conditional knockout mice (Lrp4^GFAP-Cre), astrocytic-specific Lrp4 conditional knockout mice (Lrp4^GFAP-creER), and their control mice (Lrp4^f/f) were subjected to photothrombotic ischemia and the transient middle cerebral artery occlusion. After ischemia/stroke, mice or their brain samples were subjected to behavior tests, brain histology, immunofluoresc

SUBMITTER: Ye XC 

PROVIDER: S-EPMC5742060 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature