Public Clonotypes and Convergent Recombination Characterize the Naive CD8⁺ T-Cell Receptor Repertoire of Extremely Preterm Neonates.

ABSTRACT: Respiratory support improvements have aided survival of premature neonates, but infection susceptibility remains a predominant problem. We previously reported that neonatal mice have a rapidly evolving T-cell receptor (TCR) repertoire that impairs CD8⁺ T cell immunity. To understand the impact of prematurity on the human CD8⁺ TCR repertoire, we performed next-generation sequencing of the complementarity-determining region 3 (CDR3) from the rearranged TCR variable beta (Vβ) in sorted, naïve CD8⁺ T cells from extremely preterm neonates (23-27 weeks gestation), term neonates (37-41 weeks gestation), children (16-56 months), and adults (25-50 years old). Strikingly, preterm neonates had an increased frequency of public clonotypes shared between unrelated indivi

SUBMITTER: Carey AJ 

PROVIDER: S-EPMC5742125 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature