Riemerella anatipestifer Type IX Secretion System Is Required for Virulence and Gelatinase Secretion.

ABSTRACT: Riemerella anatipestifer (RA), a major causative agent of septicemia anserum exsudativa in domesticated ducklings, has a protein secretion system known as the type IX secretion system (T9SS). It is unknown whether the T9SS contributes to the virulence of RA through secretion of factors associated with pathogenesis. To answer this question, we constructed an RA mutant deficient in sprT, which encodes a core protein of the T9SS. Deletion of sprT yielded cells that failed to digest gelatin, an effect that was rescued via complementation by a plasmid encoding wild-type sprT. Complement-mediated killing was significantly increased in the deletion mutant, suggesting that proteins secreted by the T9SS are necessary for complement evasion in RA. Liquid chromatography-ta

SUBMITTER: Guo Y 

PROVIDER: S-EPMC5742166 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature