Differentiation-Dependent Motility-Responses of Developing Neural Progenitors to Optogenetic Stimulation.

ABSTRACT: During neural tissue genesis, neural stem/progenitor cells are exposed to bioelectric stimuli well before synaptogenesis and neural circuit formation. Fluctuations in the electrochemical potential in the vicinity of developing cells influence the genesis, migration and maturation of neuronal precursors. The complexity of the in vivo environment and the coexistence of various progenitor populations hinder the understanding of the significance of ionic/bioelectric stimuli in the early phases of neuronal differentiation. Using optogenetic stimulation, we investigated the in vitro motility responses of radial glia-like neural stem/progenitor populations to ionic stimuli. Radial glia-like neural stem cells were isolated from CAG^loxpStop^loxpChR2(H134)-eYFP<

SUBMITTER: Kohidi T 

PROVIDER: S-EPMC5742229 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature