Real-time RT-PCR for Mayaro virus detection in plasma and urine.

ABSTRACT:

Background

Mayaro virus (MAYV) causes an acute febrile illness which can be difficult to differentiate from dengue or chikungunya. MAYV RNA can be detected in plasma during the first 3-5days of illness, but only a single rRT-PCR has been fully evaluated in the literature.

Objectives

To develop an rRT-PCR for MAYV and evaluate assay performance using human plasma and urine samples spiked with different MAYV strains.

Study design

A MAYV rRT-PCR targeting a region of the 5'UTR and nsp1 gene was designed from the alignment of all complete-genome MAYV sequences to be compatible with existing laboratory protocols. The assay was evaluated using human samples spiked with six MAYV strains, including strains from each of the three genotypes.

Results

The linear range of t

SUBMITTER: Waggoner JJ 

PROVIDER: S-EPMC5742299 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature