Iron enhances the peptidyl deformylase activity and biofilm formation in Staphylococcus aureus.

ABSTRACT: Staphylococcus aureus plays a major role in persistent infections and many of these species form structured biofilms on different surfaces which is accompanied by changes in gene expression profiles. Further, iron supplementation plays a critical role in the regulation of several protein(s)/enzyme function, which all aid in the development of active bacterial biofilms. It is well known that for each protein, deformylation is the most crucial step in biosynthesis and is catalyzed by peptidyl deformylase (PDF). Thus, the aim of the current study is to understand the role of iron in biofilm formation and deformylase activity of PDF. Hence, the PDF gene of S. aureus ATCC12600 was PCR amplified using specific primers and sequenced, followed by cloning and expression in Escherichia coli DH5α. Th

SUBMITTER: Swarupa V 

PROVIDER: S-EPMC5742566 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature