Diversification of the Histone Acetyltransferase GCN5 through Alternative Splicing in Brachypodium distachyon.

ABSTRACT: The epigenetic modulatory SAGA complex is involved in various developmental and stress responsive pathways in plants. Alternative transcripts of the SAGA complex's enzymatic subunit GCN5 have been identified in Brachypodium distachyon. These splice variants differ based on the presence and integrity of their conserved domain sequences: the histone acetyltransferase domain, responsible for catalytic activity, and the bromodomain, involved in acetyl-lysine binding and genomic loci targeting. GCN5 is the wild-type transcript, while alternative splice sites result in the following transcriptional variants: L-GCN5, which is missing the bromodomain and S-GCN5, which lacks the bromodomain as well as certain motifs of the histone acetyltransferase domain. Absolute mRNA

SUBMITTER: Martel A 

PROVIDER: S-EPMC5743026 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature