Zika Virus Infects, Activates, and Crosses Brain Microvascular Endothelial Cells, without Barrier Disruption.

ABSTRACT: Zika virus (ZIKV) has been associated to central nervous system (CNS) harm, and virus was detected in the brain and cerebrospinal fluids of microcephaly and meningoencephalitis cases. However, the mechanism by which the virus reaches the CNS is unclear. Here, we addressed the effects of ZIKV replication in human brain microvascular endothelial cells (HBMECs), as an in vitro model of blood brain barrier (BBB), and evaluated virus extravasation and BBB integrity in an in vivo mouse experimental model. HBMECs were productively infected by African and Brazilian ZIKV strains (ZIKV_MR766 and ZIKV_PE243), which induce increased production of type I and type III IFN, inflammatory cytokines and chemokines. Infection with ZIKV_MR766 promoted earlier cellul

SUBMITTER: Papa MP 

PROVIDER: S-EPMC5743735 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature