Promoter mutagenesis for fine-tuning expression of essential genes in Mycobacterium tuberculosis.

ABSTRACT: A range of regulated gene expression systems has been developed for mycobacteria in the last few years to facilitate the study of essential genes, validate novel drug targets and evaluate their vulnerability. Among these, the TetR/Pip-OFF repressible promoter system was successfully used in several mycobacterial species both in vitro and in vivo. In the first version of the system, the repressible promoter was P_ptr , a strong Pip-repressible promoter of Streptomyces pristinaespiralis, which might hamper effective downregulation of genes with a low basal expression level. Here, we report an enhanced system that allows more effective control of genes expressed at low level. To this end, we subjected P_ptr to targeted mutagenesis and produced 16 different promoters with d

SUBMITTER: Boldrin F 

PROVIDER: S-EPMC5743821 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature