Estrogen and high-fat diet induced alterations in C57BL/6 mice endometrial transcriptome profile.

ABSTRACT: Unopposed estrogen stimulation and insulin resistance are known to play important roles in endometrial cancer (EC), but the interaction between these two factors and how they contribute to endometrial lesions are not completely elucidated. To investigate the endometrial transcriptome profile and the associated molecular pathway alterations, we established an ovariectomized C57BL/6 mouse model treated with subcutaneous implantation of 17-β estradiol (E2) pellet and/or high-fat diet (HFD) for 12 weeks to mimic sustained estrogen stimulation and insulin resistance. Histomorphologically, we found that both E2 and E2 + HFD groups showed markedly enlarged uterus and increased number of endometrial glands. The endometrium samples were collected for microarray assay. GO and KEGG analysis showed th

SUBMITTER: Cheng Y 

PROVIDER: S-EPMC5744625 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature