Convergent evolution and topologically disruptive polymorphisms among multidrug-resistant tuberculosis in Peru.

ABSTRACT:

Background

Multidrug-resistant tuberculosis poses a major threat to the success of tuberculosis control programs worldwide. Understanding how drug-resistant tuberculosis evolves can inform the development of new therapeutic and preventive strategies.

Methods

Here, we use novel genome-wide analysis techniques to identify polymorphisms that are associated with drug resistance, adaptive evolution and the structure of the phylogenetic tree. A total of 471 samples from different patients collected between 2009 and 2013 in the Lima suburbs of Callao and Lima South were sequenced on the Illumina MiSeq platform with 150bp paired-end reads. After alignment to the reference H37Rv genome, variants were called using standardized methodology. Genome-wide analysis was undertaken using cus

SUBMITTER: Grandjean L 

PROVIDER: S-EPMC5744980 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature