Library Construction from Subnanogram DNA for Pelagic Sea Water and Deep-Sea Sediments.

ABSTRACT: Shotgun metagenomics is a low biased technology for assessing environmental microbial diversity and function. However, the requirement for a sufficient amount of DNA and the contamination of inhibitors in environmental DNA leads to difficulties in constructing a shotgun metagenomic library. We herein examined metagenomic library construction from subnanogram amounts of input environmental DNA from subarctic surface water and deep-sea sediments using two library construction kits: the KAPA Hyper Prep Kit and Nextera XT DNA Library Preparation Kit, with several modifications. The influence of chemical contaminants associated with these environmental DNA samples on library construction was also investigated. Overall, shotgun metagenomic libraries were constructed from 1 pg to 1 ng of input DN

SUBMITTER: Hirai M 

PROVIDER: S-EPMC5745018 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature