Detection of bovine mastitis pathogens by loop-mediated isothermal amplification and an electrochemical DNA chip.

ABSTRACT: Bovine mastitis causes significant economic losses in the dairy industry. Effective prevention of bovine mastitis requires an understanding of the infection status of a pathogenic microorganism in a herd that has not yet shown clinical signs of mastitis and appropriate treatment specific for the pathogenic microorganism. However, bacterial identification by culture has drawbacks in that the sensitivity may be low and the procedure can be complex. In this study, we developed a genetic detection method to identify mastitis pathogens using a simple and highly sensitive electrochemical DNA chip which can specifically detect bacterial DNA in milk specimens. First, we selected microorganisms belonging to 12 families and/or genera associated with mastitis for which testing should be performed. Ne

SUBMITTER: Kawai K 

PROVIDER: S-EPMC5745174 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature