Visualization of Peripheral Neuron Sensitization in a Surgical Mouse Model of Osteoarthritis by In Vivo Calcium Imaging.

ABSTRACT:

Objective

To develop a method for analyzing sensory neuron responses to mechanical stimuli in vivo, and to evaluate whether these neuronal responses change after destabilization of the medial meniscus (DMM).

Methods

DMM or sham surgery was performed in 10-week-old male C57BL/6 wild-type or Pirt-GCaMP3^+/- mice. All experiments were performed 8 weeks after surgery. Knee and hind paw hyperalgesia were assessed in wild-type mice. The retrograde label DiI was injected into the ipsilateral knee to quantify the number of knee-innervating neurons in the L4 dorsal root ganglion (DRG) in wild-type mice. In vivo calcium imaging was performed on the ipsilateral L4 DRG of Pirt-GCaMP3^+/- mice as mechanical stimuli (paw pinch, knee pinch, or knee twist) were applied t

SUBMITTER: Miller RE 

PROVIDER: S-EPMC5745281 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature