A uniform-shear rate microfluidic bioreactor for real-time study of proplatelet formation and rapidly-released platelets.

ABSTRACT: Platelet transfusions, with profound clinical importance in blood clotting and wound healing, are entirely derived from human volunteer donors. Hospitals rely on a steady supply of donations, but these methods are limited by a 5-day shelf life, the potential risk of contamination, and differences in donor/recipient histocompatibility. These challenges invite the opportunity to generate platelets ex vivo. Although much progress has been made in generating large numbers of culture-derived megakaryocytes (Mks, the precursor cells to platelets), stimulating a high percentage of Mks to undergo platelet release remains a major challenge. Recent studies have demonstrated the utility of shear forces to enhance platelet release from cultured Mks. In this study, we performed a computational fluid dy

SUBMITTER: Martinez AF 

PROVIDER: S-EPMC5745287 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature