B cells require Type 1 interferon to produce alloantibodies to transfused KEL-expressing red blood cells in mice.

ABSTRACT:

Background

Alloantibodies to red blood cell (RBC) antigens can cause significant hemolytic events. Prior studies have demonstrated that inflammatory stimuli in animal models and inflammatory states in humans, including autoimmunity and viremia, promote alloimmunization. However, molecular mechanisms underlying these findings are poorly understood. Given that Type 1 interferons (IFN-α/β) regulate antiviral immunity and autoimmune pathology, the hypothesis that IFN-α/β regulates RBC alloimmunization was tested in a murine model.

Study design and methods

Leukoreduced murine RBCs expressing the human KEL glycoprotein were transfused into control mice (WT), mice lacking the unique IFN-α/β receptor (IFNAR1^-/- ), or bone marrow chimeric mice lacking IFNAR1 on specific ce

SUBMITTER: Gibb DR 

PROVIDER: S-EPMC5745367 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature