ORFeome-based identification of biomarkers for serodiagnosis of Mycobacterium tuberculosis latent infection.

ABSTRACT:

Background

The challenges posed by Mycobacterium tuberculosis infection require the gradual removal of the pool of latent tuberculosis infection (LTBI). The current cell-immune-based diagnostic tests used to identify LTBI individuals have several irreversible drawbacks. In the present study, we attempted to identify novel diagnostic antigens for LTBI.

Methods

A high-throughput glutathione S-transferase (GST)-fusion technology was used to express over 409 TB proteins and sera from LTBI and healthy individuals was used to interrogate these GST-TB fusion proteins.

Results

Of 409 TB proteins, sixty-three reacted seropositive and defined the immuno-ORFeome of latent M. tuberculosis. Within the immuno-ORFeome, the rare targets were predominantly latency-associated proteins

SUBMITTER: Zhou F 

PROVIDER: S-EPMC5745629 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature