Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression.

ABSTRACT:

Background

In recent years, the concern of Acanthamoeba keratitis has increased since the infection is often associated with contact lens use. Partial 18S rRNA genotypic identification of Acanthamoeba isolates is important to correlate with pathophysiological properties in order to evaluate the degree of virulence. This is the first report of genotypic identification for clinical isolates of Acanthamoeba from corneal scrapings of keratitis in Malaysia. This study is also the first to correlate the mRNA expression of MBP and AhLBP as virulent markers for axenic strains of Acanthamoeba.

Results

In this study, ten clinical isolates were obtained from corneal scrapings. Rns genotype and intra-genotypic variation at the DF3 region of the isolates were identified. Results revealed

SUBMITTER: Ng SL 

PROVIDER: S-EPMC5745754 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature