Global transcriptional landscape and promoter mapping of the gut commensal Bifidobacterium breve UCC2003.

ABSTRACT:

Background

Bifidobacterium breve represents a common member of the infant gut microbiota and its presence in the gut has been associated with host well being. For this reason it is relevant to investigate and understand the molecular mechanisms underlying the establishment, persistence and activities of this gut commensal in the host environment.

Results

The assessment of vegetative promoters in the bifidobacterial prototype Bifidobacterium breve UCC2003 was performed employing a combination of RNA tiling array analysis and cDNA sequencing. Canonical -10 (TATAAT) and -35 (TTGACA) sequences were identified upstream of transcribed genes or operons, where deviations from this consensus correspond to transcription level variations. A Random Forest analysis assigned the -10 regio

SUBMITTER: Bottacini F 

PROVIDER: S-EPMC5746004 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature